朱思军，医学博士，博士
LANGUAGES
研究项目和附属机构
研究兴趣
Genetic mechanisms of Drosophila neural stem cell specification and sub-cellular specific targeting of dendrites.
EDUCATION
研究抽象
Our lab is interested in elucidating cellular and molecular mechanisms that regulate brain development using Drosophila 作为一个模式生物. Specifically, 我们正在进行以下两项研究，利用各种强大的基因, genomic, 分子生物学, 生化和共聚焦成像方法.
1) Genetic mechanisms that control the generation of brain complexity and brain tumor formation. The increased brain complexity is believed to be responsible for higher intellectual ability in humans. Generating brain complexity requires the maintenance of neural stem cells (NSCs) and the production of intermediate neural progenitor cells (INPs), transient amplification of which is critical for boosting the number of neurons and glia generated from NSCs. However, aberrant proliferation or defects in differentiation of NSCs and INPs can lead to brain tumor formation. Drosophila II型神经母细胞(NBs) Drosophila NSCs)非常类似于哺乳动物的NSCs. They can not only maintain their own population through self-renewal but also produce large amount of neurons by generating transient amplifying INPs. Furthermore, type II NB lineages are extremely susceptible to tumorigenesis because INPs are genetically unstable and prone to dedifferentiation (Fig.1). Thus, type II NB lineages provide an excellent model for studying the generation of brain complexity and the formation of brain tumors. The major goal of this line of research is to dissect genetic mechanisms that control the specification/self-renewal of type II NBs and differentiation/dedifferentiation of INPs in the developing Drosophila 大脑的转录和转录后水平.
Fig. 1. (A-A') A normal type II NB lineage labeled with GFP (A) and schematic diagrams of the lineage and its neurogenesis pattern (A'). (B-’)具有INPs过早分化的II型NB谱系. (C-C’)II型NB谱系，INP分化有缺陷.
2) Cellular and molecular mechanisms that control neural circuit formation at the subcellular level in the Drosophila central brain. One of the fundamental questions in developmental neurobiology is how neurons form subcellular specific synaptic contacts. Subcellular-specificity of synaptic connections has a profound impact on neuronal activity and behavior output. In the Drosophila 学习记忆中心, the mushroom body, mushroom body output neurons (MBONs) target their dendrites to specific subcellular locations (or called compartments) of the mushroom body axons to form synaptic contacts. A total of 34 MBONs of 21 types elaborate their dendrites in 16 compartments that together tile the five MB axonal lobes without overlap (Fig. 2). We use the mushroom body output neurons as a novel model system to investigate cellular and molecular mechanisms that govern the compartment-specific targeting and tiling of dendrites in the central brain of Drosophila.
Fig. 2. (A)的图解 Drosophila 蘑菇体及其五个轴突叶. (B) 5个蘑菇轴突叶上16个隔室示意图. Targeting of MBON dendrites to a2 and a’2 compartments is indicated (adapted from Aso et al., 2014). (C) Two MBONs labeled in green and red target their dendrites to the γ5 and γ4 compartments without overlapping with each other in the mushroom body axonal lobes (in blue). (D) MBON dendrites labeled with alternative fluorescent colors tile four neighboring compartments (γ2 through γ5) of the mushroom body γ lobe.
提交稿件
康立明，谢勇，陈锐，朱生. Kin17 drives dissociation of Mira from the centrosome in neuroblasts by regulating splicing of Flfl. bioRxiv 2021.11.03.467193; doi: http://doi.org/10.1101/2021.11.03.467193(修订于 Cell Reports)
选定的出版物
陈锐，邓鑫，朱思. The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating Tailless. PLoS Genet. 2022年1月,18 (1):e1009928. doi: 10.1371/journal.pgen.1009928. 收集2022年1月. PubMed PMID: 35100262; PubMed Central PMCID: PMC8830786.
陈锐，侯毅，康奈明，朱思. Homeodomain protein Six4 prevents the generation of supernumerary Drosophila type II neuroblasts and premature differentiation of intermediate neural progenitors. PLoS Genet. 2021年2月,17 (2):e1009371. doi: 10.1371/journal.pgen.1009371. 藏品2021年2月. PubMed PMID: 33556050; PubMed Central PMCID: PMC7895384.
朱松，陈锐，索巴平，Jan yyn. JNK信号与蜕皮激素信号协同促进植物的修剪 Drosophila 感觉神经元树突. Development. 2019年4月17日;146(8). doi: 10.1242/dev.163592. PubMed PMID: 30936183; PubMed Central PMCID: PMC6503988.
Rode S, Ohm H, Anhäuser L, Wagner M, Rosing M, Deng X, Sin O, Leidel SA, Storkebaum E, Rentmeister A, Zhu S, Rumpf S. Differential Requirement for Translation Initiation Factor Pathways during Ecdysone-Dependent Neuronal Remodeling in Drosophila. Cell Rep. 2018年8月28日;24(9):2287-2299.e4. doi: 10.1016/j.celrep.2018.07.074. PubMed PMID: 30157424.
李旭，陈锐，朱思. bHLH-O proteins balance the self-renewal and differentiation of Drosophila neural stem cells by regulating Earmuff expression. Dev Biol. 2017年11月15日;431(2):239-251. doi: 10.1016/j.ydbio.2017.09.011. Epub 2017 Sep 9. PubMed PMID: 28899667; PubMed Central PMCID: PMC5658246.
谢燕，李翔，邓翔，侯燕，O'Hara K, Urso A，彭燕，陈玲，朱思. The Ets protein Pointed prevents both premature differentiation and dedifferentiation of Drosophila intermediate neural progenitors. Development. 2016年9月1日;143(17):3109-18. doi: 10.1242/dev.137281. Epub 2016 Aug 10. PMID:27510969
李旭，谢宇，朱思. Notch maintains Drosophila type II neuroblasts by suppressing expression of the Fez transcription factor Earmuff. Development. 2016年7月15日;143(14):2511-21. doi: 10.1242/dev.136184. Epub 2016 May 5.PMID:27151950
Huang X, He Y, Dubuc AM, Hashizume R, Zhang W, Reimand J, Yang H, Wang TA, Stehbens SJ, Younger S, Barshow S, Zhu S, Cooper MK, Peacock J, Ramaswamy V, Garzia L, Wu X, Remke M, Forester CM, Kim CC, Weiss WA, James CD, Shuman MA, Bader GD, Mueller S, Taylor MD, Jan YN, Jan LY. EAG2 potassium channel with evolutionarily conserved function as a brain tumor target. Nat Neurosci. 2015年9月,18(9):1236 - 46所示. doi: 10.1038/nn.4088. Epub 2015 Aug 10. PubMed PMID: 26258683; PubMed Central PMCID: PMC4639927.
谢勇，李翔，张翔，梅思，李宏，乌尔索阿，朱思. The Drosophila Sp8 transcription factor Buttonhead prevents premature differentiation of intermediate neural progenitors. Elife. 2014 Oct 6;3. doi: 10.7554/eLife.03596.PMID:25285448
韩超，王东，Soba P，朱松，林旭，Jan LY, Jan YN. Integrins regulate repulsion-mediated dendritic patterning of drosophila sensory neurons by restricting dendrites in a 2D space. Neuron. [j] .吉林大学学报(自然科学版);33 (1):464 - 468. doi: 10.1016/j.neuron.2011.10.036.PMID:22243747
朱松，朱秀杰，王冬杰，杨丽丽，杨勇. Ets transcription factor Pointed promotes the generation of intermediate neural progenitors in Drosophila larval brains. 美国国家科学基金委. 2011年12月20日;08(5):20615-20. doi: 10.1073/pnas.1118595109. Epub 2011 Dec 5.PMID:22143802
索巴P，朱生，Emoto K, Younger S，杨士杰，余海红，李涛，Jan LY, Jan YN. Drosophila sensory neurons require Dscam for dendritic self-avoidance and proper dendritic field organization. Neuron. 2007年5月3日;54(3):403-16.PMID:17481394
朱松，林松，高春春，江志强，李涛. Gradients of the Drosophila Chinmo BTB-zinc finger protein govern neuronal temporal identity. Cell. [j] .中国科学院学报(自然科学版);2006;27(2):491 - 491.PMID:17055440
郭春涛，朱思，杨少森，杨丽娟. Identification of E2/E3 ubiquitinating enzymes and caspase activity regulating Drosophila sensory neuron dendrite pruning. Neuron. 2006年8月3日;51(3):283-90.PMID:16880123
朱思，潘明，李涛. Requirement of Cul3 for axonal arborization and dendritic elaboration in Drosophila mushroom body neurons. J Neurosci. 2005年4月20日;25(16):4189-97.PMID:15843622